缓冲液中血小板α-颗粒成分与14C-去甲肾上腺素相关的自发流出。

Spontaneous efflux of platelet alpha-granule components in relation to 14C-norepinephrine in buffers.

作者信息

Stavenow L, Mattiasson I, Theodorsson B, Hood B

出版信息

Life Sci. 1982 May 31;30(22):1899-905. doi: 10.1016/0024-3205(82)90470-2.

Abstract

In an investigation of the spontaneous efflux of beta-thromboglobulin (beta-TG), 14C-norepinephrine (14C-NE) and growth stimulating activity (GSA), measured as incorporation of 3H-thymidine in rabbit aortic smooth cells, from platelets resuspended in Ca++- and Mg++-free buffer a continuous increase in 3H-thymidine incorporation was seen during 120 min. There was good stoichiometric parallelism between beta-TG and 14C-NE during the 80 minutes the experiment lasted, indicating that norepinephrine is incorporated in alpha-granules and release by exocytosis.

摘要

在一项关于β-血小板球蛋白（β-TG）、14C-去甲肾上腺素（14C-NE）和生长刺激活性（以3H-胸腺嘧啶核苷掺入兔主动脉平滑肌细胞来衡量）自血小板的自发流出的研究中，将血小板重悬于无Ca++和Mg++的缓冲液中，在120分钟内观察到3H-胸腺嘧啶核苷掺入持续增加。在实验持续的80分钟内，β-TG和14C-NE之间存在良好的化学计量平行关系，表明去甲肾上腺素被掺入α-颗粒并通过胞吐作用释放。