Walker J E, Ogston D
Thromb Haemost. 1982 Jun 28;47(3):265-8.
Plasma reduced the activity of urokinase and, to a lesser extent, tissue activator on unheated fibrin plates. After depletion of plasminogen by lysine-Sepharose the inhibitory effect of plasma was increased. Normal plasma separated on DEAE-Sepharose or, after passage through lysine-Sepharose, on Sephadex G-100 provided fractions in three regions capable of inhibiting tissue activator. The high molecular weight fractions inhibited both activators, but after plasma fractionation on Sephadex 4B the inhibitory activities against tissue activator and urokinase eluted separately. Urokinase inhibition eluted with alpha 2-macroglobulin: the inhibition of tissue activator was of higher molecular weight and of greater lability. Inhibition of tissue activator was found in two lower molecular weight regions: the fractions of one region inhibited tissue activator and coincided with the elution of fast-acting antiplasmin activity. The other region inhibited both tissue activator and urokinase. Alpha1-antitrypsin and antithrombin III eluted after both inhibitory regions.
血浆可降低未加热纤维蛋白平板上尿激酶的活性，对组织型纤溶酶原激活物（t-PA）活性的降低程度较小。用赖氨酸-琼脂糖去除纤溶酶原后，血浆的抑制作用增强。在二乙氨基乙基琼脂糖（DEAE-琼脂糖）上分离的正常血浆，或在通过赖氨酸-琼脂糖后在葡聚糖凝胶G-100上分离的正常血浆，可在三个区域得到能够抑制t-PA的组分。高分子量组分可抑制两种激活物，但在葡聚糖凝胶4B上对血浆进行分级分离后，对t-PA和尿激酶的抑制活性分别洗脱。尿激酶抑制活性与α2-巨球蛋白一起洗脱：对t-PA的抑制作用分子量更高且更不稳定。在两个较低分子量区域发现了对t-PA的抑制作用：一个区域的组分抑制t-PA，且与快速作用的抗纤溶酶活性的洗脱一致。另一个区域则同时抑制t-PA和尿激酶。α1-抗胰蛋白酶和抗凝血酶III在两个抑制区域之后洗脱。
