Kok P
Thromb Haemost. 1979 Jun 30;41(4):734-44.
Normal human plasma contains acid-stable as well as labile plasminogen activators. The activity of activators in plasma euglobulins was inhibited by EACA in an uniform pattern, similar to that obtained with the major activators in human uterine tissue or with the purified porcine tissue activator, but different from the patterns obtained with plasmin or with urokinase. Gel filtration at high ionic strength separated activators corresponding to particle sizes of 60,000 dalton and about 10,000 dalton, corresponding to two activators similarly obtained from human tissue. The 60,000 dalton activator was precipitated in the euglobulin fraction. Its concentration increased in plasma after exercise. The 10,000 dalton activator was found mainly in the supernatant. Gel filtration in 0.15 M solutions yielded activators in fractions of molecular sizes of 100-140,000 dalton and 200,000 dalton or larger. The activity of normal and exercise euglobulins was inhibited by antiserum to a plasminogen activator prepared from porcine tissue, but it was not inhibited by antiserum to urokinase. Plasminogen activators in human plasma euglobulins resembled immunochemically the activators in human uterine tissue.
正常人血浆中含有酸稳定和不稳定的纤溶酶原激活剂。血浆优球蛋白中激活剂的活性被6 -氨基己酸以一种均匀的模式抑制，这与在人子宫组织中的主要激活剂或纯化的猪组织激活剂所获得的模式相似，但与纤溶酶或尿激酶所获得的模式不同。在高离子强度下进行凝胶过滤分离出对应于60,000道尔顿和大约10,000道尔顿颗粒大小的激活剂，这与从人体组织中类似获得的两种激活剂相对应。60,000道尔顿的激活剂沉淀在优球蛋白部分。运动后其在血浆中的浓度升高。10,000道尔顿的激活剂主要存在于上清液中。在0.15 M溶液中进行凝胶过滤，在分子大小为100 - 140,000道尔顿和200,000道尔顿或更大的部分中产生激活剂。正常和运动优球蛋白的活性被针对从猪组织制备的纤溶酶原激活剂的抗血清抑制，但不被抗尿激酶抗血清抑制。人血浆优球蛋白中的纤溶酶原激活剂在免疫化学上类似于人子宫组织中的激活剂。
