Sedloev N, Unia F, de Armas M
Vet Med Nauki. 1982;19(1):104-9.
Electrophoresis of the investigated materials was carried out in a Shandou SAE soothern 2761 chamber. The amylase activity was demonstrated by the sandwich method on an agar starch substrate. A comparatively best result was obtained with the use of a Celogel carrier, followed by the cellulose-acetate folio and agar. It was found that the rumen liquid had up to 5 amylase-active zones with the use of Celogel or cellulose-acetate folio. The proper conditions for incubation of the substrate were: temperature--45 degrees C, time--1 h, and pH value--5.
在所研究材料的电泳实验中，使用汕头SAE南方2761型电泳槽进行。淀粉酶活性通过在琼脂淀粉底物上的夹心法来显示。使用Celogel载体获得了相对最佳的结果，其次是醋酸纤维素薄片和琼脂。结果发现，使用Celogel或醋酸纤维素薄片时，瘤胃液有多达5个淀粉酶活性区。底物孵育的适宜条件为：温度——45摄氏度，时间——1小时，pH值——5。
