Skude G
Scand J Clin Lab Invest. 1975 Jan;35(1):41-47. doi: 10.1080/00365517509068003.
An electrophoretic technique for demonstrating amylase isoenzymes is described. After separation in an agarose gel containing a linear polyacrylamide polymer to reduce electroendosmotic flow, the amylase fractions are visualized by incubation with a commercially available dye-starch polymer (Phadebas Amylase Test). The technique detects amylase fractions with activities below 10 U/l. Some characteristic changes in such diseases as acute and chronic pancreatitis, cystic fibrosis of the pancreas, macroamylasemia and inherited variants as well as after maxillofacial surgery are mentioned.
本文描述了一种用于展示淀粉酶同工酶的电泳技术。在含有线性聚丙烯酰胺聚合物的琼脂糖凝胶中进行分离以减少电渗流后，通过与市售染料 - 淀粉聚合物（法德巴斯淀粉酶试验）孵育使淀粉酶组分可视化。该技术可检测活性低于10 U/l的淀粉酶组分。文中提到了急性和慢性胰腺炎、胰腺囊性纤维化、巨淀粉酶血症、遗传变异以及颌面手术后等疾病中的一些特征性变化。
