Reddehase M J, Cox J H, Koszinowski U H
Eur J Immunol. 1982 Jun;12(6):519-23. doi: 10.1002/eji.1830120613.
The aim of this study was to determine the number and state of activity of cytolytic T lymphocytes (CTL) and their precursors (CTL-P) present in vivo during the early stages of viral infection. The local response to lethal infection with rabies virus was used as a model system that is not accessible to analysis by secondary activation in vitro. The local response to alloantigen served as a control. Experimental protocols were established that allow frequency estimates of in vivo antigen-triggered CTL-P. Data allow a distinction between CTL-P activated in vivo by alloantigen and viral antigen with respect to their different capacity to utilize T cell growth factors (interleukins). In vivo alloantigen-primed CTL-P generate, in vitro, an active effector progeny in the presence of interleukins of xenogeneic origin, whereas the majority of virus-specific CTL-P, in spite of considerable expansion in vivo, fail to generate CTL in vitro unless antigen is added.
本研究的目的是确定在病毒感染早期体内存在的细胞毒性T淋巴细胞（CTL）及其前体（CTL-P）的数量和活性状态。以对狂犬病病毒致死性感染的局部反应作为一个模型系统，该系统无法通过体外二次激活进行分析。对同种异体抗原的局部反应作为对照。建立了实验方案，可对体内抗原触发的CTL-P进行频率估计。数据表明，体内由同种异体抗原和病毒抗原激活的CTL-P在利用T细胞生长因子（白细胞介素）方面能力不同。体内经同种异体抗原致敏的CTL-P在体外异种来源的白细胞介素存在下可产生有活性的效应子代，而大多数病毒特异性CTL-P尽管在体内有相当程度的扩增，但除非添加抗原，否则在体外无法产生CTL。
