Cooke B A, Golding M, Dix C J, Hunter M G
Mol Cell Endocrinol. 1982 Jul;27(2):221-31. doi: 10.1016/0303-7207(82)90111-3.
The effects of the beta-adrenergic hormone agonist, isoproterenol, on testosterone and cyclic AMP production in mouse Leydig cells in culture have been investigated. It was found that isoproterenol increased testosterone production on days 1, 2 and 3 of culture but not in freshly cultured cells. Cyclic AMP production was however increased on all days of culture. In subsequent studies carried out on day 2 of culture the amounts of testosterone formed during incubation with isoproterenol were 20-90% of those obtained with maximum stimulating levels of luteinizing hormone. The amounts of cyclic AMP formed were extremely low compared with those obtained with luteinizing hormone (22 +/- 5.3 and 2320 +/- 100 pmoles/10(6) cells/2 h respectively). Isoproterenol (10(-8) -10(-7) M) gave a significant increase in testosterone production and reached a maximum with 10(-6) M. Similar dose-response curves for cyclic AMP production were obtained. The stimulation of cyclic AMP and testosterone by isoproterenol was highly dependent on the presence of the phosphodiesterase inhibitor, methylisobutylxanthine. Propranolol blocked, in a dose-dependent manner, both isoproterenol-stimulated cyclic AMP and testosterone production. In the presence of excess luteinizing hormone no additional effects of isoproterenol were detected. Epinephrine also stimulated testosterone production. It is concluded that catecholamines stimulate testosterone production in mouse Leydig cells in monolayer culture and that this effect if mediated by cyclic AMP.
研究了β - 肾上腺素能激素激动剂异丙肾上腺素对培养的小鼠睾丸间质细胞中睾酮和环磷酸腺苷（cAMP）生成的影响。结果发现，异丙肾上腺素在培养的第1、2和3天增加了睾酮的生成，但对新鲜培养的细胞没有此作用。然而，在培养的所有天数中环磷酸腺苷的生成均增加。在培养第2天进行的后续研究中，与用最大刺激水平的促黄体生成素孵育时相比，与异丙肾上腺素孵育期间形成的睾酮量为其20 - 90%。与用促黄体生成素获得的量相比，形成的环磷酸腺苷量极低（分别为22±5.3和2320±100皮摩尔/10⁶个细胞/2小时）。异丙肾上腺素（10⁻⁸ - 10⁻⁷M）使睾酮生成显著增加，在10⁻⁶M时达到最大值。环磷酸腺苷生成也获得了类似的剂量 - 反应曲线。异丙肾上腺素对环磷酸腺苷和睾酮的刺激高度依赖于磷酸二酯酶抑制剂甲基异丁基黄嘌呤的存在。普萘洛尔以剂量依赖的方式阻断异丙肾上腺素刺激的环磷酸腺苷和睾酮生成。在存在过量促黄体生成素的情况下，未检测到异丙肾上腺素的额外作用。肾上腺素也刺激睾酮生成。结论是，儿茶酚胺刺激单层培养的小鼠睾丸间质细胞中睾酮的生成，且这种作用是由环磷酸腺苷介导的。
