Instituto de Histología y Embriología (IHEM)-CONICET, Facultad de Ciencias Médicas, Cuyo Medical School, Universidad Nacional de Cuyo, Post Box 56, 5500 Mendoza, Argentina.
Endocrine. 2012 Apr;41(2):266-73. doi: 10.1007/s12020-011-9568-9. Epub 2011 Dec 7.
The autonomic nerve fibres converge to the testis along two major pathways, the superior spermatic nerve (SSN) and the inferior spermatic nerve (ISN). The object of this work was to evaluate whether the addition of noradrenaline (NA) in the ganglionic compartment of two ex vivo systems: superior mesenteric ganglion (SMG)-SSN-testis, inferior mesenteric ganglion (IMG)-ISN-testis modulate androstenedione (A₂), NA and nitrite release and to determine whether there are secretory differences between the right and the left testis. Each gonad with its respective ganglion was transferred into a cuvette with two compartments and incubated in a Dubnoff metabolic shaker. The testis incubation liquids were collected and analysed for NA by HPLC, A₂ by RIA and nitrites by the Griess method. When NA is added to the IMG, A₂ and NA release diminishes and nitrite increases in the left testis, while in the right gonad, A₂ and NA increase and nitrite decreases. When NA was administered to the SMG, A₂ and NA increase and nitrite diminishes in the left gonad, but they show opposite fluctuations in the right testis. These ex vivo systems appear to be excellent models for studying the sympathetic ganglionic control of the testis though A₂, NA and nitrite release from the male gonad. It is evident that a better knowledge about the role of catecholamines and nitric oxide in the testis physiology may facilitate the understanding of some reproductive diseases.
自主神经纤维通过两条主要途径汇聚到睾丸，即精索上神经（SSN）和精索下神经（ISN）。本研究旨在评估去甲肾上腺素（NA）在两个离体系统的神经节腔中的添加是否会调节睾丸酮（A₂）、NA 和亚硝酸盐的释放，以及是否存在左右睾丸之间的分泌差异。每个带有其相应神经节的睾丸被转移到一个具有两个隔室的比色皿中，并在杜诺夫代谢摇床中孵育。收集睾丸孵育液，通过 HPLC 分析 NA，通过 RIA 分析 A₂，通过 Griess 法分析亚硝酸盐。当将 NA 添加到 IMG 时，左睾丸中的 A₂和 NA 释放减少，而亚硝酸盐增加，而在右侧睾丸中，A₂和 NA 增加，亚硝酸盐减少。当 NA 被给予 SMG 时，左睾丸中的 A₂和 NA 增加，而亚硝酸盐减少，但在右侧睾丸中表现出相反的波动。这些离体系统似乎是研究交感神经节对睾丸控制的理想模型，通过这些模型可以研究 A₂、NA 和亚硝酸盐从男性睾丸中的释放。显然，更好地了解儿茶酚胺和一氧化氮在睾丸生理学中的作用可能有助于理解一些生殖疾病。
