Kasturi K, Hannoun C
Ann Microbiol (Paris). 1977 Apr;128A(3):349-64.
The development of local and systemic cell mediated immunity (CMI) was investigated in rabbits after intratracheal immunization with live or inactivated influenza A virus. Lymphocytes from bronchoalveolar washings and draining lymph nodes were used for the assay of local CMI response. Spleen cells were employed for determination of systemic responses. Since alveolar macrophages were found to be susceptible to the action of migration inhibiting factor, the development of CMI in lungs was assayed by macrophage migration inhibition test using bronchoalveolar was cells. Immunization with live virus induces beter local CMI response than inactivated virus. After primary immunization the peak responses were found between second and third week. The secondary response was detectable much earlier and peaked at one week after booster. Small amounts of inactivates virus, which was unsufficient to evoke a primary response, could elicit a strong secondary response. The development of rapid and accentuated secondary response in the local lymphoid tissues suggests the presence of memory in the local CMI response. The results also show a good correlation to exist between primary local CMI response and the skin reactivity to the immunizing antigen. However, lack of such correlation during the secondary response leaves the skin tests less reliable in the evaluation of CMI in viral infections. Some of the functions of alveolar macrophages in the local immune reponses are also discussed.
在用活的或灭活的甲型流感病毒经气管内免疫家兔后，研究了局部和全身细胞介导免疫（CMI）的发展情况。支气管肺泡灌洗物和引流淋巴结中的淋巴细胞用于检测局部CMI反应。脾细胞用于测定全身反应。由于发现肺泡巨噬细胞易受迁移抑制因子的作用，因此使用支气管肺泡灌洗细胞通过巨噬细胞迁移抑制试验来检测肺中CMI的发展。用活病毒免疫比用灭活病毒诱导出更好的局部CMI反应。初次免疫后，在第二周和第三周之间发现了峰值反应。二次反应在更早的时候就可检测到，并在加强免疫后一周达到峰值。少量不足以引发初次反应的灭活病毒可引发强烈的二次反应。局部淋巴组织中快速且增强的二次反应的发展表明局部CMI反应中存在记忆。结果还表明，初次局部CMI反应与对免疫抗原的皮肤反应性之间存在良好的相关性。然而，二次反应期间缺乏这种相关性使得皮肤试验在评估病毒感染中的CMI时不太可靠。还讨论了肺泡巨噬细胞在局部免疫反应中的一些功能。
