DL-天冬氨酸对无细胞液体培养基中鼠麻风分枝杆菌生长的刺激作用。

Stimulation of growth of Mycobacterium lepraemurium in cell-free liquid medium by DL-aspartic acid.

作者信息

Nakamura M

出版信息

Int J Lepr Other Mycobact Dis. 1982 Jun;50(2):193-9.

Abstract

The growth of M. lepraemurium in cell-free liquid medium was strongly stimulated by addition of DL-aspartic acid at a final concentration of 0.01% to 0.02% to ND and ND-5 media containing dextran and liposome. No effect of DL-aspartic acid was observed when it was added to ND and ND-5 media without dextran and liposome. Optimal pH of the culture medium is critical for the stimulating effect of DL-aspartic acid, and it varies with the composition of the medium; the optimal pH was 6.0 in ND medium containing dextran and liposome (NDLA), and was 6.6 in NDLA medium supplemented with alpha-ketoglutarate, 1-cysteine HCl, hemin, and cytochrome c (NDLA-5). A possible mechanism of the effect of aspartic acid is discussed.

摘要

在含有葡聚糖和脂质体的ND和ND - 5培养基中添加终浓度为0.01%至0.02%的DL - 天冬氨酸，可强烈刺激鼠麻风杆菌在无细胞液体培养基中的生长。当将DL - 天冬氨酸添加到不含葡聚糖和脂质体的ND和ND - 5培养基中时，未观察到其有任何作用。培养基的最适pH值对DL - 天冬氨酸的刺激作用至关重要，且随培养基组成而异；在含有葡聚糖和脂质体的ND培养基（NDLA）中，最适pH值为6.0，而在添加了α - 酮戊二酸、半胱氨酸盐酸盐、血红素和细胞色素c的NDLA培养基（NDLA - 5）中，最适pH值为6.6。文中讨论了天冬氨酸作用的一种可能机制。