体外培养的鼠麻风分枝杆菌中的α-酮戊二酸脱氢酶

Alpha-ketoglutarate dehydrogenase in the in vitro-grown Mycobacterium lepraemurium.

作者信息

Ishaque M, Togola D, Sticht-Groh V

机构信息

Applied Microbiology Research Center, Institut Armand-Frappier, University of Quebec, Laval, Canada.

出版信息

Int J Lepr Other Mycobact Dis. 1994 Sep;62(3):399-403.

PMID:7963913

Abstract

The Hawaiian and Kumato strains of Mycobacterium lepraemurium were cultivated on Ogawa egg-yolk medium, and the alpha-ketoglutarate dehydrogenase activity was investigated in cell-free preparations of this mycobacterium. The enzymatic activity was mainly localized in the particulate fraction (150,000 x g pellet), and extremely low activity was found in the soluble fraction (150,000 x g supernatant). alpha-Ketoglutarate dehydrogenase was not stable; the activity was lost completely when the enzyme was kept at 45 degrees C for 1 hr or stored at -70 degrees C. The enzyme reduced only NAD+ but not NADP+ by alpha-ketoglutarate, indicating the presence of NAD(+)-dependent alpha-ketoglutarate dehydrogenase in cultivated M. lepraemurium.

摘要

将夏威夷株和熊本株的鼠麻风分枝杆菌在小川蛋黄培养基上培养，并对该分枝杆菌的无细胞制剂中的α-酮戊二酸脱氢酶活性进行了研究。酶活性主要定位于颗粒部分（150,000×g沉淀），而在可溶性部分（150,000×g上清液）中发现极低的活性。α-酮戊二酸脱氢酶不稳定；当酶在45℃保持1小时或在-70℃储存时，活性完全丧失。该酶仅通过α-酮戊二酸还原NAD + 而不还原NADP + ，表明在培养的鼠麻风分枝杆菌中存在NAD(+)依赖性α-酮戊二酸脱氢酶。