Wharton W, Pledger W J
J Cell Biochem. 1982;19(1):17-26. doi: 10.1002/jcb.240190103.
Concentrations of methylglyoxal bis-(guanylhydrazone) (mGBG) that inhibited serum-stimulated BALB/c-3T3 cells in late G1 caused a marked inhibition of 3H-leucine incorporation during a 20-min incubation. No decrease was observed in the incorporation of 3H-uridine during a 20-min incubation; however, the amount of acid-insoluble 3H-uridine in mGBG-treated cultures was decreased when the incubation period was longer than 20 min. The amount of the decrease in the accumulation of incorporated 3H-uridine was directly proportional to the length of the incorporation time. Between 10 and 12 h after quiescent BALB/c-3T3 cells were serum-stimulated in mGBG no additional 3H-uridine was accumulated. The stability of the incorporated 3H-uridine, as determined by acid-insoluble radioactivity remaining after the addition of actinomycin D, was less in cells cultured in mGBG. Exogenous spermine or spermidine reversed the inhibition of 3H-uridine accumulation in acid-insoluble material produced by mGBG as well as the decrease in stability of the incorporated 3H-uridine in acid-insoluble material. The effects of mGBG on both the incorporation of 3H-uridine and the stability of the incorporated 3H-uridine can apparently be accounted for by an effect on ribosomal RNA.
抑制血清刺激的BALB/c - 3T3细胞在G1晚期的甲基乙二醛双（胍腙）（mGBG）浓度，在20分钟的孵育期间导致3H - 亮氨酸掺入的显著抑制。在20分钟的孵育期间未观察到3H - 尿苷掺入的减少；然而，当孵育时间长于20分钟时，mGBG处理的培养物中酸不溶性3H - 尿苷的量减少。掺入的3H - 尿苷积累减少的量与掺入时间的长度成正比。在mGBG中血清刺激静止的BALB/c - 3T3细胞后10至12小时，没有额外的3H - 尿苷积累。通过放线菌素D添加后剩余的酸不溶性放射性确定的掺入的3H - 尿苷的稳定性，在mGBG培养的细胞中较低。外源性精胺或亚精胺逆转了mGBG产生的酸不溶性物质中3H - 尿苷积累的抑制以及酸不溶性物质中掺入的3H - 尿苷稳定性的降低。mGBG对3H - 尿苷掺入和掺入的3H - 尿苷稳定性的影响显然可以通过对核糖体RNA的影响来解释。
