Boynton A L, Whitfield J F, Isaacs R J
J Cell Physiol. 1976 Nov;89(3):481-8. doi: 10.1002/jcp.1040890313.
Changing the medium, or adding fresh serum, induces a large proportion of the proliferatively quiescent cells in confluent monolayers of human WI-38 and mouse BALB/3T3 cells to initiate a growth-division cycle. Exposure at the time of the medium change or serum addition to MGBG (methyl glyoxal bis [guanylhydrazone]), an inhibitor of spermidine and spermine synthesis and function, reduces or stops the subsequent flow of cells into the DNA-synthetic phase, without grossly affecting RNA synthesis. Mediation of MGBG action by an actual or functional shortage of spermidine or spermine (but not putrescine), and consequently an involvement of these polyamines in DNA synthesis, is strongly suggested by the reduction of the inhibitor's effectiveness by a brief (1-hour), early prereplicative exposure of the treated cells to exogenous spermidine and spermine (but not putrescine).
更换培养基或添加新鲜血清，可诱导人WI - 38和小鼠BALB / 3T3细胞汇合单层中大部分增殖静止细胞进入生长分裂周期。在更换培养基或添加血清时，将细胞暴露于MGBG（甲基乙二醛双[胍腙]），一种亚精胺和精胺合成及功能的抑制剂，可减少或阻止随后细胞进入DNA合成期，而对RNA合成无明显影响。经短暂（1小时）、复制前早期将处理过的细胞暴露于外源性亚精胺和精胺（而非腐胺）后，抑制剂的有效性降低，这有力地表明MGBG的作用是由亚精胺或精胺（而非腐胺）的实际或功能性缺乏介导的，因此这些多胺参与了DNA合成。
