McKenzie J L, Dalchau R, Fabre J W
J Neurochem. 1982 Nov;39(5):1461-6. doi: 10.1111/j.1471-4159.1982.tb12592.x.
The F10-44-2 monoclonal antibody was originally shown to interact with a determinant found predominantly in human brain and leucocytes. In this study we demonstrate by quantitative absorption analysis with homogenates of the head of the caudate nucleus, putamen, thalamus, cerebral grey matter, cerebral white matter, corpus callosum and cerebellar folia that the determinant is restricted to the white matter of the CNS. Immunofluorescence studies on frozen sections of the above brain subregions confirm the absorption analyses, showing staining only of white matter. In addition, and unexpectedly, we found very bright staining around blood vessels, particularly in the cerebellum. Biochemical studies established that the molecule in white matter bearing the F10-44-2 determinants is a sialylated membrane glycoprotein with an apparent molecular weight of 90,000, which is similar to but slightly smaller than the T lymphocyte form of the antigen. Developmental studies comparing 16-week foetal and adult cerebrum showed a fivefold increase in F10-44-2 antigen content. Thus, in the human CNS, the F10-44-2 antigen is a medium-sized glycoprotein which is restricted to white matter and shows a marked increase in concentration during development. No such molecule has been described previously.
F10 - 44 - 2单克隆抗体最初被证明可与主要在人脑和白细胞中发现的一种决定簇相互作用。在本研究中，我们通过对尾状核头部、壳核、丘脑、大脑灰质、大脑白质、胼胝体和小脑叶片匀浆进行定量吸收分析表明，该决定簇仅限于中枢神经系统的白质。对上述脑区冰冻切片的免疫荧光研究证实了吸收分析结果，显示仅白质有染色。此外，出乎意料的是，我们发现血管周围有非常明亮的染色，尤其是在小脑中。生化研究表明，白质中带有F10 - 44 - 2决定簇的分子是一种唾液酸化膜糖蛋白，表观分子量为90,000，与该抗原的T淋巴细胞形式相似但略小。对16周胎儿和成人脑进行的发育研究表明，F10 - 44 - 2抗原含量增加了五倍。因此，在人类中枢神经系统中，F10 - 44 - 2抗原是一种中等大小的糖蛋白，仅限于白质，并且在发育过程中浓度显著增加。此前尚未描述过此类分子。
