Miller C A, Benzer S
Proc Natl Acad Sci U S A. 1983 Dec;80(24):7641-5. doi: 10.1073/pnas.80.24.7641.
A panel of 146 monoclonal antibodies (MAbs), obtained with Drosophila melanogaster tissue as primary immunogen, was tested for cross-reactivity with the human central nervous system. Sites examined included spinal cord, cerebellum, hippocampus, and optic nerve. Nonnervous tissues tested were liver and lymph node. Approximately half of the antibodies reacted with one or more sites in the human central nervous system, identifying regional, cell class, and subcellular antigens. Some recognized neuronal, glial, or axonal subsets. Immunoblot analysis revealed that some antibodies reacted with similar antigen patterns in both species.
用黑腹果蝇组织作为主要免疫原获得了一组146种单克隆抗体（MAb），检测了它们与人中枢神经系统的交叉反应性。检测的部位包括脊髓、小脑、海马体和视神经。检测的非神经组织为肝脏和淋巴结。大约一半的抗体与人中枢神经系统中的一个或多个部位发生反应，识别出区域、细胞类型和亚细胞抗原。一些抗体识别神经元、神经胶质或轴突亚群。免疫印迹分析表明，一些抗体在两种物种中与相似的抗原模式发生反应。
