Aitken R J, Holme E, Richardson D W, Hulme M
J Reprod Fertil. 1982 Sep;66(1):327-34. doi: 10.1530/jrf.0.0660327.
Intact and univalent antibodies were prepared against mechanically isolated mouse zonae pellucidae solubilized in a variety of ways (heat, low pH, SDS, urea and trypsin). The antisera bound avidly and specifically to solubilized iodinated zona antigens and the intact zona structure. When the concentrations of immunoreactive Fab material in the intact and univalent antibody preparations were equalized and compared for their ability to block the sperm-binding stage of fertilization, only the intact gamma-globulin preparations possessed antifertility activity. These results indicate that antibodies raised against intact solubilized zonae pellucidae block fertilization by cross-linking antigens on the outer zona surface, thereby indirectly masking the sperm receptor sites. The integrity of these surface components did not appear to be affected by solubilization procedures that disrupt non-covalent bonds (heating, low pH, SDS and urea) although they did appear to be adversely affected by trypsin treatment. None of the antisera tested contained antibodies directed against the sperm receptor site indicating that these critical components lack immunogenicity.
制备了针对以多种方式（加热、低pH值、十二烷基硫酸钠、尿素和胰蛋白酶）溶解的机械分离的小鼠透明带的完整和单价抗体。抗血清能强烈且特异性地结合溶解的碘化透明带抗原和完整的透明带结构。当使完整和单价抗体制剂中免疫反应性Fab物质的浓度相等，并比较它们阻断受精过程中精子结合阶段的能力时，只有完整的γ球蛋白制剂具有抗生育活性。这些结果表明，针对完整溶解的透明带产生的抗体通过交联透明带外表面的抗原，从而间接掩盖精子受体位点来阻断受精。这些表面成分的完整性似乎不受破坏非共价键的溶解程序（加热、低pH值、十二烷基硫酸钠和尿素）的影响，尽管它们似乎受到胰蛋白酶处理的不利影响。所测试的抗血清均不含有针对精子受体位点的抗体，这表明这些关键成分缺乏免疫原性。
