精子受体活性与纯化猪透明带抗原（PPZA）的关联。

Association of sperm receptor activity with a purified pig zona antigen (PPZA).

作者信息

Sacco A G, Subramanian M G, Yurewicz E C

出版信息

J Reprod Immunol. 1984 Feb;6(2):89-103. doi: 10.1016/0165-0378(84)90003-2.

Abstract

Three types of data are presented which suggest that sperm receptor activity is associated with a 58,000 Mr acidic glycoprotein (PPZA) isolated from pig zonae: (1) boar sperm fail to bind to pig zonae pretreated with univalent antibody to PPZA; (2) boar sperm pretreated with PPZA are inhibited in binding to intact pig zonae; (3) binding studies utilizing boar sperm and radiolabeled PPZA indicate an inhibition of radiolabeled PPZA binding to sperm by non-labeled PPZA in a dose-related manner. Collectively these data suggest that, at least in the pig system, the contraceptive action of zona antibodies is accomplished by a specific immunologic reaction between antibody and the zona sperm receptor site rather than to a non-specific blockage of this receptor.

摘要

呈现了三种类型的数据，这些数据表明精子受体活性与从猪透明带中分离出的一种58,000道尔顿的酸性糖蛋白（PPZA）有关：（1）公猪精子无法与用PPZA单价抗体预处理过的猪透明带结合；（2）用PPZA预处理过的公猪精子与完整猪透明带的结合受到抑制；（3）利用公猪精子和放射性标记的PPZA进行的结合研究表明，未标记的PPZA以剂量相关的方式抑制放射性标记的PPZA与精子的结合。总体而言，这些数据表明，至少在猪的系统中，透明带抗体的避孕作用是通过抗体与透明带精子受体位点之间的特异性免疫反应来实现的，而不是通过对该受体的非特异性阻断。