小鼠β2-微球蛋白同种异型的分离与鉴定。

Isolation and characterization of mouse beta 2-microglobulin allotypes.

作者信息

Graf L, Peterson P A, Sege K, Robinson P J

出版信息

Mol Immunol. 1982 Jul;19(7):877-84. doi: 10.1016/0161-5890(82)90353-4.

Abstract

Two allelic forms of mouse beta 2-microglobulin (beta 2m), the smaller polypeptide chain of the H-2 histocompatibility antigens, were purified from urine and partially characterized. The isoelectric points of the two allotypes are 7.4 (beta 2ma) and 8.1 (beta 2mb). The electrophoretic mobility of beta 2ma is decreased by reduction, whereas the mobility of beta 2mb is not. Urinary beta 2m can replace endogenous beta 2m in mouse H-2 histocompatibility antigens.

摘要

从小鼠尿液中纯化出了H-2组织相容性抗原较小的多肽链——小鼠β2-微球蛋白（β2m）的两种等位基因形式，并对其进行了部分特性鉴定。这两种同种异型的等电点分别为7.4（β2ma）和8.1（β2mb）。β2ma的电泳迁移率在还原后降低，而β2mb的迁移率则不受影响。尿液中的β2m可替代小鼠H-2组织相容性抗原中的内源性β2m。