抗肿瘤抗生素博来霉素对构巢曲霉的重组和诱变作用。
Recombinogenic and mutagenic effects of the antitumor antibiotic bleomycin in Aspergillus nidulans.
作者信息
Demopoulos N A, Kappas A, Pelecanos M
出版信息
Mutat Res. 1982 Jul-Aug;102(1):51-7. doi: 10.1016/0165-1218(82)90145-8.
Bleomycin, an antibiotic and antineoplastic drug that inhibits DNA synthesis and causes several types of chromosomal aberration, was found to increase mitotic recombination in Aspergillus nidulans. Heterozygous prototrophic diploid strains grown on media containing bleomycin produced significant increases of yellow and white sectors compared with controls. Further, the increased colour segregants were due to mitotic crossing-over, whereas the non-dis junctional segregants remained at the control level. Bleomycin also induced point mutations in the methionine-suppressor system of the methGl biAl strain of Aspergillus nidulans. Conidia treated in suspension with various concentrations of bleomycin increased the methionine-independent mutants 30-fold and more.
博来霉素是一种抗生素和抗肿瘤药物，可抑制DNA合成并导致多种类型的染色体畸变，人们发现它能增加构巢曲霉中的有丝分裂重组。与对照组相比，在含有博来霉素的培养基上生长的杂合原养型二倍体菌株产生的黄色和白色扇形区域显著增加。此外，颜色分离体的增加是由于有丝分裂交换，而非分离的分离体则保持在对照水平。博来霉素还在构巢曲霉的methGl biAl菌株的甲硫氨酸抑制系统中诱导点突变。用不同浓度的博来霉素悬浮处理分生孢子，使不依赖甲硫氨酸的突变体增加了30倍以上。
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