Stephanou G, Demopoulos N A
University of Patras, Department of Biology, Greece.
Curr Genet. 1986;10(11):791-6. doi: 10.1007/BF00418524.
Heat shock was found to induce characteristic changes in the pattern of protein synthesis in Aspergillus nidulans as analysed by SDS-polyacrylamide gel electrophoresis. Six to seven new bands were found to show increased incorporation to 35S-methionine at 43 degrees C compared to 37 degrees C, the standard temperature for this organism. The heat shock response of five different strains of A. nidulans was examined. This comparative study showed that these strains (haploids and diploids) show exactly the same set of heat shock proteins.
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析发现,热休克可诱导构巢曲霉蛋白质合成模式发生特征性变化。与该生物体的标准温度37℃相比,在43℃时发现有6至7条新条带显示出35S-甲硫氨酸掺入增加。研究了构巢曲霉五个不同菌株的热休克反应。这项比较研究表明,这些菌株(单倍体和二倍体)显示出完全相同的一组热休克蛋白。