Somogyi P, Takagi H
Neuroscience. 1982 Jul;7(7):1779-83. doi: 10.1016/0306-4522(82)90035-5.
The buffered picric acid paraformaldehyde fixative originally recommended for electronmicroscopy and which has since been used occasionally for light-microscopic immunocytochemistry, has been supplemented with glutaraldehyde and used as primary fixative for the perfusion of rat brains. In the basal ganglia and preoptic area, substance P, somatostatin and leu-enkephalin immunoreactive material was localized with the unlabelled antibody enzyme method in thick sections cut from freeze-thaw treated blocks. Good penetration of the antibodies without the use of detergents and the light background of the osmium-treated sections allowed the selection for electron-microscopy of immunoreactive structures as small as individual boutons that had been identified at the light-microscopic level. It is suggested that the procedure may be useful for electron-microscopic sampling of immunoreactive structures occurring infrequently over a large area or for the electron-microscopic study of light-microscopically classified neurons.
最初推荐用于电子显微镜检查、此后偶尔用于光学显微镜免疫细胞化学的缓冲苦味酸多聚甲醛固定剂，已添加戊二醛并用作大鼠脑灌注的初次固定剂。在基底神经节和视前区，采用未标记抗体酶法，在经冻融处理的组织块切出的厚切片中定位P物质、生长抑素和亮脑啡肽免疫反应性物质。在不使用去污剂的情况下抗体具有良好的穿透性，且经锇处理的切片背景较淡，这使得能够选择光学显微镜水平鉴定出的小至单个终扣的免疫反应性结构用于电子显微镜检查。有人认为，该方法可能适用于对大面积中不常出现的免疫反应性结构进行电子显微镜取样，或用于对光学显微镜分类的神经元进行电子显微镜研究。
