Bowker R M, Westlund K N, Sullivan M C, Wilber J F, Coulter J D
Peptides. 1982 May-Jun;3(3):291-8. doi: 10.1016/0196-9781(82)90089-4.
The localization of serotonergic and various peptidergic neurons in the medullary raphe nuclei that project to the lumbosacral spinal cord have been studied using a retrograde transport method combined with immunocytochemistry. Spinally projecting neurons stained for serotonin-like, substance P-like, enkephalin-like and thyrotropin-releasing hormone-like immunoreactivity were all observed in the raphe nuclei of the medulla, as well as in the adjacent ventrolateral reticular formation. The distribution of the descending serotonergic and peptidergic neurons in the raphe nuclei as well as quantitative data on their relative numbers suggest that a large fraction of raphe-spinal neurons contain serotonin co-existing with one or more peptides in the same cell.
利用逆行运输法结合免疫细胞化学技术，对投射至腰骶脊髓的延髓中缝核内的5-羟色胺能神经元和各种肽能神经元进行了定位研究。在延髓的中缝核以及相邻的腹外侧网状结构中，均观察到了呈5-羟色胺样、P物质样、脑啡肽样和促甲状腺激素释放激素样免疫反应性染色的脊髓投射神经元。中缝核内下行5-羟色胺能和肽能神经元的分布及其相对数量的定量数据表明，大部分中缝脊髓神经元在同一细胞内含有5-羟色胺并与一种或多种肽共存。
