Gordon J W, Ruddle F H
Prog Clin Biol Res. 1982;85 Pt B:111-24.
Our laboratory has recently demonstrated the successful transfer of genetic material into the genomes of newborn mice by injection of that material into pronuclei of fertilized eggs. Our initial results indicated two patterns of processing of the injected DNA: one in which the material was not integrated into the host genome, and another in which the injected genes became associated with high molecular weight DNA. We now present evidence that these patterns are maintained through further development to adulthood. We provide definitive evidence of the covalent association of injected DNA with host sequences, and transmission of such linked sequences in a Mendelian distribution to 2 succeeding generations of progeny. The observation that injected gene sequences can become integrated and stably transmitted through the germ line should contribute significantly to further studies of this gene transfer system.
我们实验室最近通过将遗传物质注射到受精卵的原核中，成功地将其转移到新生小鼠的基因组中。我们的初步结果表明，注射的DNA有两种处理模式：一种是该物质未整合到宿主基因组中，另一种是注射的基因与高分子量DNA相关联。我们现在提供证据表明，这些模式在进一步发育到成年期的过程中得以维持。我们提供了注射的DNA与宿主序列共价结合的明确证据，以及这种连锁序列以孟德尔方式传递给两代后代的证据。注射的基因序列能够通过种系整合并稳定传递这一观察结果，应为该基因转移系统的进一步研究做出重大贡献。
