生物技术在治疗感音神经性听力损失中的应用：毛细胞再生的基础与未来。
Biotechnology in the treatment of sensorineural hearing loss: foundations and future of hair cell regeneration.
机构信息
Emerson College, Boston, MA, USA.
出版信息
J Speech Lang Hear Res. 2011 Dec;54(6):1709-31. doi: 10.1044/1092-4388(2011/10-0149). Epub 2011 Mar 8.
PURPOSE
To provide an overview of the methodologies involved in the field of hair cell regeneration. First, the author provides a tutorial on the biotechnological foundations of this field to assist the reader in the comprehension and interpretation of the research involved in hair cell regeneration. Next, the author presents a review of stem cell and gene therapy and provides a critical appraisal of their application to hair cell regeneration. The methodologies used in these approaches are highlighted.
METHOD
The author conducted a narrative review of the fields of cellular, molecular, and developmental biology, tissue engineering, and stem cell and gene therapy using the PubMed database.
RESULTS
The use of biotechnological approaches to the treatment of hearing loss--approaches such as stem cell and gene therapy-has led to new methods of regenerating cochlear hair cells in mammals.
CONCLUSIONS
Incredible strides have been made in assembling important pieces of the puzzle that comprise hair cell regeneration. However, mammalian hair cell regeneration using stem cell and gene therapy are years--if not decades--away from being clinically feasible. If the goals of the biological approaches are met, these therapies may represent future treatments for hearing loss.
目的
概述毛细胞再生领域所涉及的方法学。首先，作者提供了该领域生物技术基础的教程，以帮助读者理解和解释毛细胞再生研究。接下来，作者回顾了干细胞和基因治疗，并对其在毛细胞再生中的应用进行了批判性评估。突出了这些方法中使用的方法。
方法
作者使用 PubMed 数据库对细胞、分子和发育生物学、组织工程以及干细胞和基因治疗领域进行了叙述性综述。
结果
生物技术方法治疗听力损失的应用，如干细胞和基因治疗，已经导致了在哺乳动物中再生耳蜗毛细胞的新方法。
结论
在组装构成毛细胞再生的重要部分方面已经取得了令人难以置信的进展。然而，使用干细胞和基因治疗的哺乳动物毛细胞再生在临床上可行之前还需要数年时间——如果不是几十年的话。如果生物方法的目标得以实现，这些疗法可能代表着治疗听力损失的未来疗法。
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