Aguas A P
Stain Technol. 1982 Mar;57(2):69-73. doi: 10.3109/10520298209066530.
Secondary treatment of glutaraldehyde fixed liver samples with long term stored solutions of osmium tetroxide and potassium ferrocyanide (Os-Fe) results in tracing of the extracellular spaces of the tissue with an electron opaque substance. This substance does not diffuse across cell membranes, its formation probably being related to the progressive reduction of the OsO4 molecule by potassium ferrocyanide. The application of the present method in electron microscopy may be useful in overcoming the artifacts often induced by other tracing techniques such as vascular perfusion with peroxidase or immersion in lanthanum. Although the period of storage of the Os-Fe solution is a clear disadvantage of the method, it seems plausible to anticipate that further studies on the chemical interaction between osmium tetroxide and potassium ferrocyanide will provide us with a Os-Fe mixture having an immediate tracer effect.
用长期储存的四氧化锇和亚铁氰化钾（Os-Fe）溶液对戊二醛固定的肝脏样本进行二次处理，会使组织的细胞外间隙被一种电子不透明物质追踪。这种物质不会扩散穿过细胞膜，其形成可能与亚铁氰化钾对OsO4分子的逐步还原有关。本方法在电子显微镜中的应用可能有助于克服其他追踪技术（如用过氧化物酶进行血管灌注或浸入镧）经常引发的伪像。尽管Os-Fe溶液的储存期是该方法的一个明显缺点，但可以合理预期，对四氧化锇和亚铁氰化钾之间化学相互作用的进一步研究将为我们提供一种具有即时示踪效果的Os-Fe混合物。
