Neiss W F
Histochemistry. 1984;80(3):231-42. doi: 10.1007/BF00495771.
In aldehyde-fixed liver and renal cortex of rat and mouse several variations of postfixation with osmium tetroxide plus potassium ferrocyanide ( FeII ) were tried. Depending on the ferrocyanide concentration different staining patterns were observed in TEM. -Osmium-High Ferrocyanide [40 mM (approximately 1%) OsO4 + 36 mM (approximately 1.5%) FeII , pH 10.4], stains membranes and glycogen. Cytoplasmic ground substance, mitochondrial matrices and chromatin are partially extracted, cell surface coats remain unstained. Membrane contrast, but extraction too, are higher with solutions containing cacodylate- than phosphate-buffer. -Osmium-Low Ferrocyanide [40 mM (approximately 1%) OsO4 + 2 mM (approximately 0.08%) FeII , pH 7.4], stains cell surface coats and basal laminae, but not glycogen, except for special cases. The trilaminar structure of membranes is poorly delineated. Signs of cytoplasmic extraction are not visible. The surface coat staining is stronger and more widespread with solutions containing phosphate- instead of cacodylate-buffer; it is enhanced by section staining with lead citrate. The cell surface coat stain does not traverse tight junctions nor permeate membranes.
在大鼠和小鼠经醛固定的肝脏及肾皮质中，尝试了几种四氧化锇加亚铁氰化钾（FeII）后固定的方法。根据亚铁氰化钾的浓度不同，在透射电镜下观察到不同的染色模式。-锇-高铁氰化物[40 mM（约1%）四氧化锇 + 36 mM（约1.5%）FeII，pH 10.4]，可使膜和糖原染色。细胞质基质、线粒体基质和染色质部分被提取，细胞表面被膜未染色。含二甲胂酸盐缓冲液的溶液比磷酸盐缓冲液的膜对比度更高，但提取效果也更强。-锇-低铁氰化物[40 mM（约1%）四氧化锇 + 2 mM（约0.08%）FeII，pH 7.4]，可使细胞表面被膜和基膜染色，但特殊情况除外，糖原不被染色。膜的三层结构显示不佳。未观察到细胞质提取的迹象。含磷酸盐缓冲液而非二甲胂酸盐缓冲液的溶液对细胞表面被膜的染色更强且更广泛；用柠檬酸铅进行切片染色可增强染色效果。细胞表面被膜染色不会穿过紧密连接，也不会渗透过膜。
