Barazarte M, Conroy M C, de Weck A L
Allergy. 1982 Jul;37(5):365-71. doi: 10.1111/j.1398-9995.1982.tb01923.x.
We have examined the use of 3H-histamine for evaluating in vitro basophil responses. Incubation of 3H-histamine for 4 h with basophil-enriched mononuclear fractions containing autologous plasma, resulted in the best incorporation of the label. Even so, less than 3% of the added label was actually recovered and other leukocytes also bound or incorporated 3H-histamine. Labeled mononuclear preparations failed to release 3H-histamine when challenged with zymosan-activated serum or anti-IgE, but endogenous histamine released under the same experimental conditions could be determined fluorometrically. The calcium ionophore, A23187, however, did cause a dose-dependent release of 3H-histamine. We conclude that 3H-histamine is not preferentially incorporated into basophil granules, and that this technique cannot be used for assessing allergen and/or IgE-mediated basophil responses.
我们已经研究了使用³H-组胺来评估体外嗜碱性粒细胞反应。³H-组胺与含有自体血浆的富含嗜碱性粒细胞的单核细胞组分孵育4小时，可实现最佳的标记掺入。即便如此，实际回收的添加标记不到3%，并且其他白细胞也会结合或掺入³H-组胺。当用酵母聚糖激活的血清或抗IgE刺激时，标记的单核细胞制剂不会释放³H-组胺，但在相同实验条件下释放的内源性组胺可以用荧光法测定。然而，钙离子载体A23187确实会引起³H-组胺的剂量依赖性释放。我们得出结论，³H-组胺不会优先掺入嗜碱性粒细胞颗粒中，并且该技术不能用于评估变应原和/或IgE介导的嗜碱性粒细胞反应。
