Richardson M, Gerrity R G, Alavi M Z, Moore S
Arteriosclerosis. 1982 Sep-Oct;2(5):369-79. doi: 10.1161/01.atv.2.5.369.
We studied proteoglycan distribution in areas of spontaneously occurring high and low permeability by TEM examination of ruthenium red-stained sections of the aortic arch of normolipemic and hyperlipemic pigs. We noted granules of two sizes: those smaller than 20 nm contained heparan sulphate, and those from 20 to 50 nm in size contained chondroitin or dermatan sulphate. In the aortas of pigs fed a normal diet, there were significantly more granules of both types in low permeability areas than in areas permeable to Evans blue dye. This is consistent with the theory that glycosaminoglycan provides a component for the control of aortic permeability. In the aortas of pigs fed cholesterol, there was an accumulation of lipid-filled monocytes in areas of high permeability and an increase in proteoglycan granule concentration, suggesting an increase in glycosaminoglycan concentration, which may be the precursor to extracellular lipid deposition.
我们通过对正常血脂和高血脂猪主动脉弓的钌红染色切片进行透射电镜检查，研究了蛋白聚糖在自然发生的高通透性和低通透性区域的分布情况。我们注意到有两种大小的颗粒：小于20纳米的颗粒含有硫酸乙酰肝素，而大小在20至50纳米之间的颗粒含有硫酸软骨素或硫酸皮肤素。在喂食正常饮食的猪的主动脉中，低通透性区域中这两种类型的颗粒都明显多于伊文思蓝染料可透过的区域。这与糖胺聚糖为控制主动脉通透性提供一种成分的理论相一致。在喂食胆固醇的猪的主动脉中，高通透性区域出现了充满脂质的单核细胞积聚，并且蛋白聚糖颗粒浓度增加，这表明糖胺聚糖浓度升高，这可能是细胞外脂质沉积的前兆。
