Huang W, Richardson M, Alavi M Z, Julian J, Moore S
Atherosclerosis. 1984 Apr;51(1):59-74. doi: 10.1016/0021-9150(84)90144-8.
The morphologic response of the aortic wall of the normal fed rat to intimal injury by an indwelling catheter in place for 3 weeks was examined at the time of removal of the catheter and 4, 8, and 26 weeks later. Changes in the concentration of glycosaminoglycan were assayed morphometrically in ruthenium red-stained sections examined by transmission electron microscopy. Two types of ruthenium red-positive granules were identified in the intercellular matrix; large (greater than 20 nm), containing chondroitin sulphate and small (less than 20 nm) containing heparan sulphate. Endothelial denudation and some thrombus seen at the time of removal of the catheter, was associated with a decrease in the concentrations of both types of granules in the subendothelial space compared to normal rats. At later time intervals there was neointimal thickening containing no lipid, and occasional small areas of lipid accumulation. Compared to normal rats there was no significant change in granule concentration in the subendothelial space at any time interval. There was a slight increase in the concentration of large granules of the deep neointima at the time of removal of the catheter and after 4 weeks. In lipid-containing areas there was a highly significant increase in large granule concentrations compared to normal or to intimal thickening without lipid. The observations support the possibility that lipid accumulation is related to an increase in glycosaminoglycan concentration.
在留置导管3周后移除导管时以及4周、8周和26周后，对正常饮食大鼠的主动脉壁对内膜损伤的形态学反应进行了检查。通过透射电子显微镜检查钌红染色切片，用形态计量学方法测定糖胺聚糖浓度的变化。在细胞间基质中鉴定出两种钌红阳性颗粒；大颗粒（大于20nm），含有硫酸软骨素，小颗粒（小于20nm），含有硫酸乙酰肝素。与正常大鼠相比，导管移除时观察到的内皮剥脱和一些血栓与内皮下空间中两种颗粒的浓度降低有关。在随后的时间间隔内，出现了不含脂质的新内膜增厚，偶尔有小面积脂质积聚。与正常大鼠相比，在任何时间间隔内，内皮下空间颗粒浓度均无显著变化。在导管移除时和4周后，深层新内膜的大颗粒浓度略有增加。与正常情况或无脂质的内膜增厚相比，在含脂质区域，大颗粒浓度显著增加。这些观察结果支持脂质积聚与糖胺聚糖浓度增加有关的可能性。
