Eftimiadi C, Rialdi G
Cell Biophys. 1982 Jun-Sep;4(2-3):231-44. doi: 10.1007/BF02918314.
The heat produced by neutrophils was measured with a flow microcalorimeter. 02 consumption, ATP concentration, lactic acid production and 14CO2 production from oxidation of [1-(14)C]-glucose [6-(14)C]-glucose and [U-14C]-glucose were evaluated. Experiments were also carried out in the presence of the metabolic inhibitors, N-ethylmaleimide and NaF. Heat effects were correlated to the enthalpy change of aerobic and anaerobic glucose catabolism. Two different heat contributions related to two different nonmitochondrial 02 reduction pathways are present during the metabolic burst. Theoretical and experimental data indicate that the reducing power is derived from the catabolism of glucose both through the hexose monophosphate shunt and glycolysis.
用流动微量热计测量中性粒细胞产生的热量。评估了[1-(14)C]-葡萄糖、[6-(14)C]-葡萄糖和[U-14C]-葡萄糖氧化过程中的氧气消耗、ATP浓度、乳酸生成和14CO2生成。还在代谢抑制剂N-乙基马来酰亚胺和NaF存在的情况下进行了实验。热效应与有氧和无氧葡萄糖分解代谢的焓变相关。在代谢爆发期间，存在与两种不同的非线粒体氧还原途径相关的两种不同的热贡献。理论和实验数据表明，还原力来自葡萄糖通过磷酸己糖旁路和糖酵解的分解代谢。
