差示扫描量热法在细胞过程研究中的应用：小鼠巨噬细胞的产热和葡萄糖氧化
Application of differential scanning microcalorimetry to the study of cellular processes: heat production and glucose oxidation of murine macrophages.
作者信息
Loike J D, Silverstein S C, Sturtevant J M
出版信息
Proc Natl Acad Sci U S A. 1981 Oct;78(10):5958-62. doi: 10.1073/pnas.78.10.5958.
Abstract
Differential scanning microcalorimetry provides a noninvasive method for studying heat evolution in living cells. We used this technique to measure the heat evolved by thioglycollate broth-elicited mouse macrophages, and the effects of NaF, KCN, cycloheximide, and cytochalasins B and D on this parameter. The total heat evolved in the interval 10--37 degrees C scanned at 1 degree C min-1 ranged from 300 to 2500 X 10(-12) cal (1 Cal = 4.184 J) per cell, depending on cell density, glucose concentration, and the presence or absence of various drugs.
摘要
差示扫描量热法为研究活细胞中的热释放提供了一种非侵入性方法。我们使用该技术测量了巯基乙酸盐肉汤诱导的小鼠巨噬细胞释放的热量，以及氟化钠、氰化钾、环己酰亚胺、细胞松弛素B和D对该参数的影响。在1℃/分钟的扫描速度下，在10 - 37℃区间内每个细胞释放的总热量在300至2500×10⁻¹²卡（1卡 = 4.184焦耳）之间，这取决于细胞密度、葡萄糖浓度以及各种药物的存在与否。
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