Alves P R
Chromosoma. 1982;85(5):713-22. doi: 10.1007/BF00330783.
A mouse cell line of C57Bl/6J spontaneous melanoma (clone PG 19), and a C-type virus transformed cell line (G-8 clone 124) originating from normal Balb/c mice were used in a study of the centromeric heterochromatin region of BrdU-labelled chromosomes stained by the Giemsa pH 10.4 method. Three possible explanations for the generation of compound lateral asymmetry within the centromeric heterochromatin region of the laboratory mouse are discussed: 1) inverted translocation; 2) centric fusion followed by paracentromeric fission and 3) inversion of part of the centromeric satellite DNA. These processes could be of considerable genetic and evolutionary significance. The non-random spatial position of unstained and dark stained C-bands in BrdU-labelled diplochromosomes of endoreduplicated cells can be explained as being due to the localization of the old and new DNA chains in a unineme chromatid model. The late replicating regions are shown to be located on the inside of the half-chromatid close to the axial symmetry axis of the metaphase chromosome.
在一项研究中，使用了C57Bl/6J自发黑色素瘤小鼠细胞系（克隆PG 19）以及源自正常Balb/c小鼠的C型病毒转化细胞系（G - 8克隆124），通过吉姆萨pH 10.4法对BrdU标记的染色体着丝粒异染色质区域进行染色。讨论了实验室小鼠着丝粒异染色质区域内复合侧向不对称产生的三种可能解释：1）倒位易位；2）着丝粒融合后接着是臂间断裂；3）部分着丝粒卫星DNA倒位。这些过程可能具有相当大的遗传和进化意义。在核内复制细胞的BrdU标记双染色体中，未染色和深色染色C带的非随机空间位置可以解释为是由于单一线圈染色单体模型中旧DNA链和新DNA链的定位所致。已表明晚期复制区域位于半染色体内侧，靠近中期染色体的轴对称轴。
