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用于检测BrdU标记染色体中姐妹染色单体交换的新染色方法。

New staining method for the detection of sister-chromatid exchanges in BrdU-labelled chromosomes.

作者信息

Alves P, Jonasson J

出版信息

J Cell Sci. 1978 Aug;32:185-95. doi: 10.1242/jcs.32.1.185.

DOI:10.1242/jcs.32.1.185
PMID:81211
Abstract

A direct Giemsa technique is described for the selective staining of Brd-U-substituted chromatin. It can advantageously be used for sister-chromatid exchange analysis and for the detailed cytological study of the mouse kinetochores. Presumptive inversions within the centromeric region of chromosomes 4 and 18 in the C57Black/6J mouse are described.

摘要

本文描述了一种用于选择性染色Brd-U取代染色质的直接吉姆萨技术。它可有效地用于姐妹染色单体交换分析以及对小鼠着丝粒进行详细的细胞学研究。文中还描述了C57Black/6J小鼠4号和18号染色体着丝粒区域内可能存在的倒位情况。

相似文献

1
New staining method for the detection of sister-chromatid exchanges in BrdU-labelled chromosomes.用于检测BrdU标记染色体中姐妹染色单体交换的新染色方法。
J Cell Sci. 1978 Aug;32:185-95. doi: 10.1242/jcs.32.1.185.
2
Atomic force microscopy of human metaphase chromosomes after differential staining of sister chromatids.姐妹染色单体差异染色后人类中期染色体的原子力显微镜观察。
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Selective differential staining of sister chromatids of the facultative heterochromatic X chromosome in the female mouse.雌性小鼠中兼性异染色质X染色体姐妹染色单体的选择性差异染色。
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Mouse satellite DNA, centromere structure, and sister chromatid pairing.小鼠卫星DNA、着丝粒结构和姐妹染色单体配对。
J Cell Biol. 1986 Oct;103(4):1145-51. doi: 10.1083/jcb.103.4.1145.
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Differential inhibition of sister chromatid condensation induced by 5-azadeoxycytidine in human chromosomes.5-氮杂脱氧胞苷对人类染色体姐妹染色单体凝聚的差异性抑制作用。
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6
Mechanisms involved in differential staining of BrdU substituted chromatids or chromosome regions.BrdU 取代染色单体或染色体区域差异染色所涉及的机制。
Rom J Morphol Embryol. 1995 Jan-Jun;41(1-2):43-7.
7
Sister chromatid exchange and chromosome organization based on a bromodeoxyuridine Giemsa-C-banding technique (TC-banding).基于溴脱氧尿苷吉姆萨 C 带技术(TC 带技术)的姐妹染色单体交换与染色体组织
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Sister chromatid exchange in the centromere and centromeric area.着丝粒及着丝粒区域的姐妹染色单体交换。
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[Modified method of differential staining of sister chromatids].
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On the mechanism of differential Giemsa staining of BrdU-substituted chromatids.关于BrdU取代染色单体的吉姆萨染色差异机制
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引用本文的文献

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Detecting chromatin interactions between and along sister chromatids with SisterC.利用 SisterC 检测姐妹染色单体之间和沿姐妹染色单体的染色质相互作用。
Nat Methods. 2020 Oct;17(10):1002-1009. doi: 10.1038/s41592-020-0930-9. Epub 2020 Sep 23.
2
Effects of auxin and cytokinin on induction of sister chromatid exchanges in cultured cells of wheat (Triticum aestivum L.).生长素和细胞分裂素对小麦(Triticum aestivum L.)培养细胞姊妹染色单体交换的诱导作用。
Theor Appl Genet. 1989 Oct;78(4):521-4. doi: 10.1007/BF00290836.
3
Chromosome orientation fluorescence in situ hybridization to study sister chromatid segregation in vivo.
利用染色体定向荧光原位杂交技术研究体内姐妹染色单体的分离。
Nat Protoc. 2010 Jul;5(7):1362-77. doi: 10.1038/nprot.2010.102. Epub 2010 Jul 1.
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Identification of sister chromatids by DNA template strand sequences.通过 DNA 模板链序列识别姐妹染色单体。
Nature. 2010 Jan 7;463(7277):93-7. doi: 10.1038/nature08644. Epub 2009 Dec 16.
5
Short telomeres result in organismal hypersensitivity to ionizing radiation in mammals.端粒缩短会导致哺乳动物对电离辐射产生机体超敏反应。
J Exp Med. 2000 Dec 4;192(11):1625-36. doi: 10.1084/jem.192.11.1625.
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Scanning electron microscopy of the centromeric region of L-cell chromosomes after treatment with Hoechst 33258 combined with 5-bromodeoxyuridine.用Hoechst 33258与5-溴脱氧尿苷处理后L细胞染色体着丝粒区域的扫描电子显微镜观察。
Chromosoma. 1993 Mar;102(4):227-32. doi: 10.1007/BF00352396.
7
The structure and position of mouse centromeric heterochromatin in BrdU-labelled chromosomes and diplochromosomes stained by the pH 10.4 method.通过pH 10.4方法染色的BrdU标记染色体和双染色体中小鼠着丝粒异染色质的结构和位置。
Chromosoma. 1982;85(5):713-22. doi: 10.1007/BF00330783.
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DNA extraction during Giemsa differentiation of chromatids singly and doubly substituted with BrdU.在经5-溴脱氧尿苷单取代和双取代的染色单体吉姆萨分化过程中进行DNA提取。
Chromosoma. 1981;81(5):691-700. doi: 10.1007/BF00329580.
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Different baseline sister chromatid exchange levels in density fractionated human lymphocytes.密度分级分离的人淋巴细胞中不同的基线姐妹染色单体交换水平。
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Light and scanning electron microscopic observations on the two contrasting types of sister chromatid differential staining after ultraviolet light irradiation.紫外线照射后两种对比类型的姐妹染色单体差异染色的光镜和扫描电镜观察。
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