Fagan D L, Slaughter C A, Capra J D, Sullivan T J
J Allergy Clin Immunol. 1982 Nov;70(5):399-404. doi: 10.1016/0091-6749(82)90031-8.
Monoclonal murine antibodies to human IgE, IgG1, IgG2, IgG3, IgG4, IgM, and IgA1 were prepared and their abilities to stimulate histamine release from human peripheral blood leukocytes were investigated. As expected, anti-IgE caused 8% to 76% histamine release from the leukocytes of each of 14 nonallergic donors. In one subject anti-IgE alone had no effect, but a 15% histamine release did occur after the subsequent addition of a goat anti-mouse IgG antiserum to crosslink cell-bound anti-IgE molecules. Anti-IgG4 alone caused 5% to 59% histamine release from the cells of four of 14 donors. Subsequent challenge with goat anti-mouse IgG caused an additional release of up to 15% of the basophil histamine, but only from leukocytes of donors responsive to anti-IgG4 alone. No histamine release was detected after stimulation by monoclonal antibodies to IgG1, IgG2, IgG3, IgM, or IgA1. The results of this study indicate that monoclonal antibodies to IgG4 as well as to IgE can stimulate human peripheral blood leukocytes to release histamine. These observations support the theory that antigen-IgG4 interactions can stimulate histamine release from human basophils.
制备了针对人IgE、IgG1、IgG2、IgG3、IgG4、IgM和IgA1的单克隆鼠抗体，并研究了它们刺激人外周血白细胞释放组胺的能力。正如预期的那样，抗IgE导致14名非过敏供体中每一个供体的白细胞释放8%至76%的组胺。在一名受试者中，单独的抗IgE没有作用，但在随后加入山羊抗小鼠IgG抗血清以交联细胞结合的抗IgE分子后，发生了15%的组胺释放。单独的抗IgG4导致14名供体中的4名供体的细胞释放5%至59%的组胺。随后用山羊抗小鼠IgG进行激发，导致嗜碱性粒细胞组胺额外释放高达15%，但仅来自对单独抗IgG4有反应的供体的白细胞。用针对IgG1、IgG2、IgG3、IgM或IgA1的单克隆抗体刺激后未检测到组胺释放。本研究结果表明，针对IgG4以及IgE的单克隆抗体可以刺激人外周血白细胞释放组胺。这些观察结果支持抗原-IgG4相互作用可以刺激人嗜碱性粒细胞释放组胺的理论。
