van Toorenenbergen A W, Aalberse R C
Int Arch Allergy Appl Immunol. 1981;65(4):432-40. doi: 10.1159/000232784.
In contrast to IgE, myeloma IgG4 did not block passive sensitization of basophil leukocytes by IgE antibody. Atopic sera, selected for a high level of mite-specific IgG4, lost their sensitizing activity after they had been heated at 56 degrees C. After incubation of leukocytes with myeloma IgG4 or with sera with high levels of specific IgG4, the cells did not become responsive towards anti-IgG4 antiserum. After incubation of leukocytes with fluorescein-labelled myeloma IgE, these cells released histamine with antifluorescein antiserum. In contrast, after incubation with fluorescein-labelled myeloma IgG4, a subsequent incubation with antifluorescein antiserum did not result in histamine release. Therefore, we cannot confirm the hypothesis of Vijay and Perelmutter that IgG4 can sensitize basophil leukocytes in vitro.
与IgE不同，骨髓瘤IgG4不能阻断IgE抗体对嗜碱性白细胞的被动致敏作用。选择具有高水平螨特异性IgG4的特应性血清，在56℃加热后失去其致敏活性。用骨髓瘤IgG4或具有高水平特异性IgG4的血清孵育白细胞后，细胞对抗IgG4抗血清无反应。用荧光素标记的骨髓瘤IgE孵育白细胞后，这些细胞与抗荧光素抗血清一起释放组胺。相反，用荧光素标记的骨髓瘤IgG4孵育后，随后与抗荧光素抗血清孵育不会导致组胺释放。因此，我们不能证实Vijay和Perelmutter提出的IgG4可在体外使嗜碱性白细胞致敏的假说。
