van Toorenenbergen A W, Aalberse R C
Int Arch Allergy Appl Immunol. 1982;67(2):117-22. doi: 10.1159/000233000.
Allergen-specific IgG4 was found in the sera from many normal, nonallergic individuals. Basophil leukocytes from donors with IgG4 antibodies to an allergen, but without IgE antibodies to the same allergen, did not release histamine when challenged with this allergen. In some cases, anti-IgG4 antiserum induced a release of histamine from the leukocytes. However, these cells also release histamine after incubation with normal rabbit serum or normal sheep serum. It is concluded that the IgG4-RAST cannot be used for the detection of IgG short-term sensitizing antibodies.
在许多正常、无过敏反应的个体血清中发现了过敏原特异性IgG4。来自对某过敏原具有IgG4抗体但对同一过敏原无IgE抗体的供体的嗜碱性白细胞，在受到该过敏原刺激时不会释放组胺。在某些情况下，抗IgG4抗血清可诱导白细胞释放组胺。然而，这些细胞在与正常兔血清或正常羊血清孵育后也会释放组胺。得出的结论是，IgG4-RAST不能用于检测IgG短期致敏抗体。
