Ido E, Matsuno T
Jpn J Med Sci Biol. 1982 Jun;35(3):87-96. doi: 10.7883/yoken1952.35.87.
Chicken alpha-fetoprotein was isolated from 12 to 13-day-old embryonic chicken serum by column chromatography on CM-Sephadex C-50. Hydroxyapatite and DEAE-Sephadex A-25. The purified protein was homogeneous based on polyacrylamide gel electrophoresis, immunoelectrophoresis and isoelectric focusing. The purified protein had the following physicochemical and immunological properties. (1) It was a glycoprotein with a single polypeptide chain. (2) The molecular weight of the protein was estimated at 71,000 by SDS-polyacrylamide gel electrophoresis. (3) The isoelectric point of the protein was 4.90. (4) The amino acid composition of the protein was similar to those of mammalian alpha-fetoproteins. (5) The protein showed no steroid-binding capacity. (6) It was immunologically distinct from mammalian alpha-fetoprotein. (7) No immunological cross-reaction was observed between the protein and chicken albumin.
通过在CM-葡聚糖凝胶C-50、羟磷灰石和二乙氨基乙基葡聚糖凝胶A-25上进行柱色谱，从12至13日龄鸡胚血清中分离出鸡甲胎蛋白。基于聚丙烯酰胺凝胶电泳、免疫电泳和等电聚焦，纯化后的蛋白质是均一的。纯化后的蛋白质具有以下物理化学和免疫学特性。（1）它是一种具有单条多肽链的糖蛋白。（2）通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳估计该蛋白质的分子量为71,000。（3）该蛋白质的等电点为4.90。（4）该蛋白质的氨基酸组成与哺乳动物甲胎蛋白的氨基酸组成相似。（5）该蛋白质不显示类固醇结合能力。（6）它在免疫学上与哺乳动物甲胎蛋白不同。（7）在该蛋白质与鸡白蛋白之间未观察到免疫交叉反应。
