Lai P C, Hay D M, Peters E H, Lorscheider F L
Biochim Biophys Acta. 1977 Jul 22;493(1):201-9. doi: 10.1016/0005-2795(77)90273-2.
Ovine alpha-fetoprotein was successfully isolated from fetal sheep serum by using rabbit anti-ovine alpha-fetoprotein linked to an agarose immunoadsorbent column. Antibody used in this affinity chromatography column was produced by immunizing a rabbit with highly purified alpha-fetoprotein-antibody complex to yield a monospecific antiserum to ovine alpha-fetoprotein. Following affinity chromatography, alpha-fetoprotein was further purified by preparative polyacrylamide disc gel electrophoresis ultimately yielding a 105-fold purification. The purified alpha-fetoprotein was homogeneous on analytical polyacrylamide disc gel electrophoresis. Ovine alpha-fetoprotein was found to be immunochemically related to human alpha-fetoprotein and to exhibit a molecular weight and amino acid composition similar to other mammalian alpha-fetoproteins.
通过使用与琼脂糖免疫吸附柱相连的兔抗羊甲胎蛋白，成功地从胎羊血清中分离出羊甲胎蛋白。用于该亲和层析柱的抗体是通过用高度纯化的甲胎蛋白 - 抗体复合物免疫兔子产生的，以产生针对羊甲胎蛋白的单特异性抗血清。经过亲和层析后，甲胎蛋白通过制备性聚丙烯酰胺圆盘凝胶电泳进一步纯化，最终得到105倍的纯化。纯化后的甲胎蛋白在分析性聚丙烯酰胺圆盘凝胶电泳上呈均一性。发现羊甲胎蛋白与人类甲胎蛋白存在免疫化学相关性，并且其分子量和氨基酸组成与其他哺乳动物的甲胎蛋白相似。
