通过与克隆的白蛋白互补DNA杂交来定量雌激素对非洲爪蟾白蛋白信使核糖核酸水平的影响。

Quantitation of estrogen effect on Xenopus laevis albumin mRNA levels by hybridization to cloned albumin cDNA.

作者信息

Philipp B W, Morgan E A, Shapiro D J

出版信息

J Biol Chem. 1982 Jul 25;257(14):8496-501.

Abstract

The isolation and characterization of a Xenopus laevis albumin cDNA clone, pUN18, is described. This clone was identified as a X. laevis albumin cDNA clone by hybridization-mRNA selection-translation and by other techniques. The clone was used in hybridizations to determine the effect of estrogen on albumin synthesis in livers of male X. laevis. We find that albumin mRNA levels remain relatively constant during the massive induction of vitellogenin mRNA synthesis and vitellogenin secretion which follow administration of estrogen to male X. laevis.

摘要

本文描述了非洲爪蟾白蛋白cDNA克隆pUN18的分离和特性鉴定。通过杂交-信使核糖核酸选择-翻译及其他技术，该克隆被鉴定为非洲爪蟾白蛋白cDNA克隆。该克隆用于杂交实验，以确定雌激素对雄性非洲爪蟾肝脏中白蛋白合成的影响。我们发现，在给雄性非洲爪蟾注射雌激素后，卵黄蛋白原信使核糖核酸合成和卵黄蛋白原分泌大量诱导期间，白蛋白信使核糖核酸水平保持相对恒定。