Bonissol C, Gilbert M, Ivanova L M
Ann Microbiol (Paris). 1978 Aug-Sep;129B(2):245-65.
A total of 135 cell lines was examined for mycoplasma contamination using two techniques: isolation and specific DNA-staining with DAPI or "Hoechst 33258". The two techniques showed similar results in 64% of the cases of cell contamination while the remainder was detected only by one or the other techniques: 12,82% by the staining technique and 23% by the isolation technique, which shows that the 2 techniques are complementary. The staining technique is very quick, easy to execute, very sensitive, and should be the method of choice to detect contamination when the mycoplasma do not grow in acellular media.
使用两种技术对总共135个细胞系进行支原体污染检测：分离培养法以及用4',6-二脒基-2-苯基吲哚（DAPI）或“Hoescht 33258”进行特异性DNA染色法。在64%的细胞污染案例中，这两种技术显示出相似的结果，而其余案例仅通过其中一种技术检测到：12.82%通过染色技术检测到，23%通过分离培养技术检测到，这表明这两种技术具有互补性。染色技术非常快速、易于操作、灵敏度高，当支原体在无细胞培养基中不生长时，它应该是检测污染的首选方法。
