Serum-plasma separator in amylase assay.

A modification of the Amylochrome method for amylase activity is described and evaluated. The modification consists of using Sure-Sep II Serum-Plasma Separators for the separation of the supernatant and precipitant in this procedure. Thirty patient sera and 20 control sera were run in duplicate with and without the separators. Comparison studies of the reference and separator methods show that the separator does not interfere with amylase activity, and there is excellent correlation (r = 0.99) between the methods. The time saved during centrifugation allows for easier and faster determination of amylase activity.