杜邦acaα淀粉酶检测方法的评估。

Evaluation of the Du Pont aca alpha-amylase procedure.

作者信息

James G P, Passey R B, Fuller J B, Giles M L

出版信息

Clin Chem. 1977 Mar;23(3):546-50.

Abstract

The procedure used with the Du Pont aca for alpha-amylase (1,4,-alpha-D-glucan glucanohydrolase, EC 3.2.1.1) was evaluated in our laboratory and compared with the Roche Diagnostics "Amylochrome" and Perkin-Elmer Coleman 91 amylase assays. The within-run coefficients of variation (CV) for samples of fresh normal sera were: aca 5.8% and 4.3% on two different lots of reagent, Amylochrome 7.4%, and Coleman 91 3.3%. In sera with abnormally high amylase activity, the respective CV's were: aca, 1.2% and 0.8%; Amylochrome, 2.0%; and Coleman 91, 2.8%. Day-to-day precision studies on fresh and lyophilized normal and abnormal sera gave CV's in the following ranges: aca, 1.8% to 6.7%; Amylochrome, 3.0% to 5.2%; and Coleman 91, 4.5% to 5.9%. Results by the aca procedure were linearly related to activity to about 10-fold the upper limit of normal amylase activity. For serum, correlations were: r = 0.977 for aca vs. Coleman 91 and r = 0.974 for aca vs. Amylochrome. For urine they were: r = 0.978 for aca vs. Coleman 91 and r = 0.975 for aca vs. Amylochrome. Mean recovery from 53 supplemented samples was 98%. Icterus, hemolysis, and lipemia did not interfere with method correlation of aca vs. Coleman 91 or Amylochrome.

摘要

我们实验室评估了用于杜邦aca检测α-淀粉酶（1,4-α-D-葡聚糖葡聚糖水解酶，EC 3.2.1.1）的方法，并将其与罗氏诊断公司的“Amylochrome”和珀金埃尔默公司的科尔曼91淀粉酶检测方法进行了比较。新鲜正常血清样本的批内变异系数（CV）为：aca在两批不同试剂上分别为5.8%和4.3%，Amylochrome为7.4%，科尔曼91为3.3%。在淀粉酶活性异常高的血清中，各自的CV分别为：aca，1.2%和0.8%；Amylochrome，2.0%；科尔曼91，2.8%。对新鲜和冻干的正常及异常血清进行的日间精密度研究得出的CV范围如下：aca，1.8%至6.7%；Amylochrome，3.0%至5.2%；科尔曼91，4.5%至5.9%。aca检测方法的结果与活性呈线性相关，直至正常淀粉酶活性上限的约10倍。对于血清，相关性为：aca与科尔曼91相比，r = 0.977；aca与Amylochrome相比，r = 0.974。对于尿液，相关性为：aca与科尔曼91相比，r = 0.978；aca与Amylochrome相比，r = 0.975。53个添加样本的平均回收率为98%。黄疸、溶血和脂血不干扰aca与科尔曼91或Amylochrome之间的方法相关性。