Serre G, Soleilhavoup J P, Delsol G, Planel H
Bull Assoc Anat (Nancy). 1982 Mar;66(192):137-49.
Twenty one peritoneal effusions from patients with cirrhosis were studied in order to characterize mesothelial cells, monocytes and their vacuolated forms. Morphological investigations were performed after Papanicolaou, M.G.G. and Giemsa stains; cytochemical studies by P.A.S., Alcian Blue pH 2,5 and Sudan Black B techniques. Seven enzymatic activities were appreciated: Naphtyl Acetate Esterase, Acid Phosphatase pH 5, beta Glucuronidase, Lactico-dehydrogénase, Malico D, NADH.D and Peroxidase pH 7,6. The free mesothelial cells show a metabolic and functional active phase in which their hydrolasic equipment suggests an intense lysosomal activity only correlated to pinocytosis. Monocytes in transudates show a morphologically micro-vacuolated macrophagic transformation of pinocytic type (elicited macrophages ?); they only become cytophagic when an acute inflammation occurs (activated macrophages ?). This paper emphasizes analogy of behaviour of mesothelial cells and monocytes in transudates, but demonstrates differences in their energetic metabolisms and endocytic characteristics.
为了对间皮细胞、单核细胞及其空泡化形式进行特征描述，对21例肝硬化患者的腹腔积液进行了研究。在进行巴氏染色、MGG染色和吉姆萨染色后进行形态学研究；通过过碘酸雪夫反应（PAS）、pH 2.5的阿尔辛蓝染色和苏丹黑B技术进行细胞化学研究。观察到七种酶活性：萘基乙酸酯酶、pH 5的酸性磷酸酶、β-葡萄糖醛酸酶、乳酸脱氢酶、苹果酸脱氢酶、NADH脱氢酶和pH 7.6的过氧化物酶。游离间皮细胞呈现出代谢和功能活跃期，其水解酶装置表明仅与胞饮作用相关的强烈溶酶体活性。漏出液中的单核细胞呈现出胞饮型的形态学微空泡化巨噬细胞转变（引发的巨噬细胞？）；只有在发生急性炎症时它们才会变成吞噬细胞（活化的巨噬细胞？）。本文强调了漏出液中间皮细胞和单核细胞行为的相似性，但证明了它们在能量代谢和内吞特征方面的差异。
