Iversen L L, Hanley M R, Sandberg B E, Lee C M, Pinnock R D, Watson S P
Ciba Found Symp. 1982(91):186-205. doi: 10.1002/9780470720738.ch11.
The ability of various related peptides and substance P analogues to compete for the binding of 3H-labelled substance P to rat brain membranes corresponds with their known biological activities, providing a simple model for studies of peptide receptors in the central nervous system. In salivary gland and brain slices substance P and related peptides stimulate the rate of incorporation of phosphatidylinositol, offering an alternative biochemical model for substance P receptor studies. Two types of receptor may be responsible for the actions of substance P on peripheral tissues: the SP-P type, where all tachykinins are approximately equally active, and the SP-E type, where eledoisin and kassinin are more potent than the other tachykinins. Alkyl esters of substance P appear to act as selective SP-P agonists.
各种相关肽和P物质类似物竞争3H标记的P物质与大鼠脑膜结合的能力与其已知的生物学活性相符，为中枢神经系统中肽受体的研究提供了一个简单模型。在唾液腺和脑切片中，P物质和相关肽刺激磷脂酰肌醇的掺入率，为P物质受体研究提供了另一种生化模型。P物质对周围组织的作用可能由两种类型的受体介导：SP-P型，所有速激肽的活性大致相同；SP-E型，蛙皮素和肛褶蛙皮素比其他速激肽更有效。P物质的烷基酯似乎作为选择性SP-P激动剂起作用。
