Kelly M J, Ronnekleiv O K, Eskay R L
Exp Brain Res. 1982;48(1):97-106. doi: 10.1007/BF00239576.
Luteinizing hormone-releasing hormone (LHRH)-neurons were localized with a LHRH antiserum (WP-1) in the medial basal hypothalamus (MBH) of the female rat using the sagittal hypothalamic slice preparation in combination with the peroxidase-antiperoxidase immunocytochemical technique of Sternberger (1979). Numerous darkly-stained perikarya were visualized in the cell-poor zone, lateral arcuate nucleus and the median eminence. The processes of these neurons contributed to the intensely-stained fiber bundle above the tuberoinfundibular sulcus. The fibers in this tract run in a rostrocaudal plane in the lateral external zone of the median eminence. Also, numerous fibers course into the internal zone of the median eminence, perpendicular to the rostrocaudal plane. Several LHRH-immunoreactive perikarya also were identified in the periventricular area caudal to the optic chiasm (retrochiasmatic area). The LHRH neurons were small (10 micron) bipolar neurons with fibers (dendrites) exiting from either pole which showed very little branching. It appears that the sagittal slice in combination with the appropriate fixation procedure and LHRH antiserum enabled us to demonstrate the presence of LHRH-immunoreactive perikarya in the MBH of the female rat.
利用矢状下丘脑切片标本并结合斯特恩伯格（1979年）的过氧化物酶-抗过氧化物酶免疫细胞化学技术，使用促黄体生成素释放激素（LHRH）抗血清（WP-1）对雌性大鼠内侧基底下丘脑（MBH）中的LHRH神经元进行定位。在细胞稀少区、外侧弓状核和正中隆起中可见大量深色染色的核周体。这些神经元的突起形成了位于漏斗结节沟上方的深色染色纤维束。该束中的纤维在正中隆起外侧外部区沿 rostrocaudal 平面走行。此外，许多纤维垂直于 rostrocaudal 平面进入正中隆起的内部区。在视交叉后方的室周区（视交叉后区）也鉴定出了几个LHRH免疫反应性核周体。LHRH神经元是小（10微米）双极神经元，其纤维（树突）从两极发出，分支很少。看来矢状切片结合适当的固定程序和LHRH抗血清使我们能够证明雌性大鼠MBH中存在LHRH免疫反应性核周体。
