Kozlowski G P, Chu L, Hostetter G, Kerdelhué B
Peptides. 1980 Spring;1(1):37-46. doi: 10.1016/0196-9781(80)90033-9.
This study utilized the preembedding immunocytochemical technique in order to identify LHRH-containing neurons in rat brain and define their ultrastructural characteristics. LHRH-containing neurons in the vertical limb of the diagonal band of Broca, medial septum, triangular nucleus of the septum and other regions were studied by taking ultrathin serial sections. These neurons had scant cytoplasm surrounding a centrally-located, spheroid, euchromatic nucleus. Neurosecretory granules were evenly distributed throughout the cell, but many tended to lie directly under the plasmalemma. The cytoplasm was organized in such a way that the most extensive portion of the rough endoplasmic reticulum was polar opposite to areas having high concentrations of Golgi complex, lysosome-like bodies, smooth endoplasmic reticulum and ribosomes. The perikarya had no axosomatic synapses but functional interaction via unspecialized appositions to the plasmalemma cannot be discounted. Many of the perikarya bore at least one cilium. Processes from immunonegative cells were occasionally observed to penetrate the cytoplasm of the LHRH perikaryon or its processes. At their points of origin, dendrites were found to be broadened processes containing many elements common to the cytoplasm: ribosomes, smooth endoplasmic reticulum, cristal and lamellar mitochondria, neurotubules, and an occasional alveolate caveola. Infrequently, some of the LHRH axons were partially myelinated. This method of studying serial-sectioned immunocytochemically-identified cells is suggested as a means of describing the cellular and subcellular characteristics of other specific peptide-containing cells.
本研究采用包埋前免疫细胞化学技术，以鉴定大鼠脑中含促性腺激素释放激素（LHRH）的神经元并确定其超微结构特征。通过制作超薄连续切片，对布罗卡斜角带垂直支、内侧隔、隔三角核及其他区域中含LHRH的神经元进行了研究。这些神经元的细胞质稀少，围绕着位于中央的球形常染色质核。神经分泌颗粒均匀分布于整个细胞，但许多颗粒倾向于直接位于质膜下方。细胞质的组织方式使得粗面内质网最广泛的部分与高尔基体复合体、溶酶体样小体、滑面内质网和核糖体浓度高的区域极性相对。胞体没有轴-体突触，但通过与质膜的非特化并置进行功能相互作用也不能排除。许多胞体至少有一根纤毛。偶尔观察到来自免疫阴性细胞的突起穿透LHRH胞体或其突起的细胞质。在其起源点，发现树突是增宽的突起，含有许多与细胞质共有的成分：核糖体、滑面内质网、嵴状和板层状线粒体、神经微管，以及偶尔出现的蜂窝状小窝。很少有一些LHRH轴突部分被髓鞘包裹。建议采用这种研究经免疫细胞化学鉴定的连续切片细胞的方法，作为描述其他特定含肽细胞的细胞和亚细胞特征的一种手段。
