用于检测人抗实验动物尿蛋白IgG抗体的放射免疫测定法的开发与应用。

Development and use of radioimmunoassays for the detection of human IgG antibodies to laboratory animal urinary proteins.

作者信息

Lee D, Edwards R G, Beeson M F, Dewdney J M

出版信息

Int Arch Allergy Appl Immunol. 1982;69(4):363-7. doi: 10.1159/000233201.

Abstract

A radioimmunoassay method for the detection of human IgG antibodies to laboratory animal urinary proteins has been developed using partially purified protein fractions prepared from crude urine extracts by gel filtration. The proteins from the four species, mouse, rat, guinea pig and rabbit, were labelled with 125I and used in an antigen binding assay employing a sepharose-linked staphylococcal protein A immunosorbent. Results, expressed as arbitrary units of specific IgG per unit serum, show antibodies to one or more species present in 57% of current animal workers compared with 6% of non-animal workers.

摘要

已开发出一种放射免疫测定方法，用于检测人类针对实验动物尿蛋白的IgG抗体，该方法使用通过凝胶过滤从粗尿提取物中制备的部分纯化蛋白组分。将来自小鼠、大鼠、豚鼠和兔子这四种动物的蛋白质用125I标记，并用于采用琼脂糖偶联葡萄球菌蛋白A免疫吸附剂的抗原结合测定。结果以每单位血清中特异性IgG的任意单位表示，显示57%的当前动物工作人员体内存在针对一种或多种动物的抗体，而非动物工作人员中这一比例为6%。