Swanson M C, Agarwal M K, Yunginger J W, Reed C E
Am Rev Respir Dis. 1984 May;129(5):844-9. doi: 10.1164/arrd.1984.129.5.844.
Of 20 serum samples from laboratory workers who experienced allergic symptoms after exposure to laboratory animals, 9 contained elevated IgE antibodies to guinea pig urine, pelt, or albumin by the radioallergosorbent test (RAST). Skin testing documented allergenic activity with all 3 guinea pig allergens. The cross-reactivity of these 3 guinea pig allergens was studied in RAST inhibition assays. Guinea pig pelt extract and urine produced dose-related inhibition in all 3 assays, but guinea pig albumin was inhibitory in only the homologous assay. Characterization by gel filtration, polyacrylamide gel electrophoresis, and isoelectric focusing demonstrated heterogeneity of the allergens. Air filter samples were taken in a guinea pig housing room, a research laboratory, and a library, using a high volume air sampler. An outdoor air filter sample was also taken. Guinea pig urine allergen activity was detected in all indoor air filter samples by RAST inhibition. Guinea pig pelt allergen activity was detected in all air filter samples, except the library and outdoors. Guinea pig albumin was not detected in any air filter sample. Sizing of airborne particles demonstrated that most guinea pig allergen activity was associated with particles of a diameter greater than 5 mu and less than 0.8 mu. Urine appears to be the major source of guinea pig allergens, and it is present in airborne particles small enough to penetrate the lower respiratory tract when inhaled.
在20名接触实验动物后出现过敏症状的实验室工作人员的血清样本中，通过放射变应原吸附试验（RAST）检测发现，9份样本中针对豚鼠尿液、皮毛或白蛋白的IgE抗体水平升高。皮肤试验证实了所有3种豚鼠过敏原均具有变应原活性。在RAST抑制试验中研究了这3种豚鼠过敏原的交叉反应性。豚鼠皮毛提取物和尿液在所有3种试验中均产生了剂量相关的抑制作用，但豚鼠白蛋白仅在同源试验中具有抑制作用。通过凝胶过滤、聚丙烯酰胺凝胶电泳和等电聚焦进行的特性分析表明，这些过敏原具有异质性。使用大容量空气采样器在豚鼠饲养室、研究实验室和图书馆采集空气过滤器样本，同时也采集了室外空气过滤器样本。通过RAST抑制试验在所有室内空气过滤器样本中检测到了豚鼠尿液过敏原活性。在除图书馆和室外之外的所有空气过滤器样本中检测到了豚鼠皮毛过敏原活性。在任何空气过滤器样本中均未检测到豚鼠白蛋白。对空气中颗粒大小的分析表明，大多数豚鼠过敏原活性与直径大于5微米且小于0.8微米的颗粒有关。尿液似乎是豚鼠过敏原的主要来源，并且它存在于足够小的空气传播颗粒中，当被吸入时能够穿透下呼吸道。
