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[植物基因组的分子结构]

[Molecular structure of plant genomes].

作者信息

Sivolap Iu M, D'iachenko L F, Novoselov A N

出版信息

Mol Biol (Mosk). 1977 Jul-Aug;11(4):877-82.

PMID:618330
Abstract

The genome structure of several species of Graminea and Drosophila was investigated by DNA renaturation method. Kinetics of DNA reassociation was studied by direct optical scanning and the data obout Cot curve were analized by an improved computer programm "Finger". Differences between structure DNA animals and plants are shown. Plant genomes have no unique fraction which exists in animal genomes. Slowly reassociating fraction in plants comprises about 20% DNA as compared with more than 60% in animal DNA. An analysis of kinetic complexity indicates that the relative content of the slowly reassociating fraction in the genome both of animal and of plants is much higher than that of the highly repeated DNA fraction.

摘要

采用DNA复性方法对几种禾本科植物和果蝇的基因组结构进行了研究。通过直接光学扫描研究了DNA重新缔合的动力学,并利用改进的计算机程序“Finger”分析了关于Cot曲线的数据。展示了动植物结构DNA之间的差异。植物基因组没有动物基因组中存在的独特部分。与动物DNA中超过60%相比,植物中缓慢重新缔合部分约占20%的DNA。动力学复杂性分析表明,动植物基因组中缓慢重新缔合部分的相对含量远高于高度重复DNA部分。

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