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[小麦胚细胞核DNA的核苷酸序列组织]

[Nucleotide sequence organization of nuclear DNA of wheat embryos].

作者信息

Filimonov N G, Martakova N A, Popov L S, Tarantul V Z, Aĭtkhozhin M A

出版信息

Biokhimiia. 1982 Jul;47(7):1198-207.

PMID:7115822
Abstract

The kinetic component composition of wheat embryo nuclear DNA was studied. It was shown that 32% of the genome consist of highly repetitive sequences. Intermediate repetitive sequences with repetitive frequency 1300 per genome constitute the bulk (52%) of the wheat embryo nuclear genome. The unique sequences constitute 12% of wheat embryo DNA. The individual families of intermediate repetitive and unique sequences were isolated; their reassociation kinetics were investigated and their kinetic complexity and repetition frequency were evaluated. Intermediate repetitive sequences 600-800 nucleotides in length were interspersed with unique sequences 800-1000 nucleotides long in the nuclear genome of wheat embryos. The linear relationship between the fragment length and the increasing amount of the zero-time binding DNA implies that 4% of the wheat embryos genome consists of palindromic sequences, which are clustered into groups.

摘要

对小麦胚细胞核DNA的动力学组分组成进行了研究。结果表明,基因组的32%由高度重复序列组成。每个基因组重复频率为1300的中度重复序列构成了小麦胚细胞核基因组的大部分(52%)。单拷贝序列占小麦胚DNA的12%。分离出了中度重复和单拷贝序列的各个家族;研究了它们的复性动力学,并评估了它们的动力学复杂度和重复频率。长度为600 - 800个核苷酸的中度重复序列与长度为800 - 1000个核苷酸的单拷贝序列在小麦胚细胞核基因组中相间排列。片段长度与零时结合DNA增加量之间的线性关系表明,小麦胚基因组的4%由回文序列组成,这些回文序列聚集成簇。

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